Composite
OmpR Siali

Part:BBa_K2235007

Designed by: Morgan Hjorth and Louise Larsson   Group: iGEM17_Stockholm   (2017-10-11)
Revision as of 15:58, 11 October 2017 by Registry (Talk | contribs)

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OmpR promoter regulating the expression of sialidase enzyme

OmpR is an osmotic pressure promoter. It can sense changes in for example sucrose concentration. A higher concentration of sucrose will lead to a higher expression of the gene after the OmpR promoter. In this biobrick the gene after OmpR is an enzyme called sialidase. This enzyme cuts sialic acid (that's for example found in mucus). The sialidase produced by this biobrick has a HIS-tag on it to make it easy to purify.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 636
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 801
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1181


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Categories
Parameters
None