Coding

Part:BBa_K2324001

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-11)
Revision as of 12:32, 11 October 2017 by Registry (Talk | contribs)

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FimH+sfGFP at residue 225

An IPTG inducible construct in BL21(DE3) which produces a modified FimH protein fused with GFP. This modified protein will involve itself in pilus biosynthesis and is placed at the very end of the pilus structures on the bacterial cell surface membrane. FimH retains its mannose binding capability, but also fluoresces.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None