Project
110-F1-F2

Part:BBa_K2491026

Designed by: Philippe Hansen-Estruch   Group: iGEM17_CCA_San_Diego   (2017-10-10)
Revision as of 20:57, 10 October 2017 by Registry (Talk | contribs)

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Combination of Part 1 and Part 2 of Fluorene Degradation - 110

Ligation of F2_XbaI_PstI into 110_F1_EcorI,SpeI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3149
    Illegal NheI site found at 3172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5049
    Illegal AgeI site found at 6061
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 403
    Illegal BsaI.rc site found at 1515
    Illegal SapI.rc site found at 5592


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