Coding

Part:BBa_K2361001

Designed by: Mart Bartelds   Group: iGEM17_Groningen   (2017-10-10)
Revision as of 09:01, 10 October 2017 by Registry (Talk | contribs)

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dCas9 VRER

This part is an adapted variant of dCas9sp. The preferred difference between dCas9 sp and dCas9 VRER is insertion of mutations resulting in a dCas9 protein recognizing a different PAM site. This was done to ensure that the appropriate binding of the protein-sgRNA complex to the complementary spacer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/crispr/cas9
Parameters
biology
protein