Measurement

Part:BBa_K2447014

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-05)
Revision as of 23:14, 9 October 2017 by David135 (Talk | contribs)


IPTG-inducible temperature-sensitive system with GFP reporter

In the absence of IPTG, lacI repressor will bind to pLac promoter and inhibit further downstream expression. pLac promoter is attached upstream of the tlpa36 protein coding sequence to control the amount of tlpa36 protein expression. When IPTG is present and under high temperatures (above 36 degree), pTlpa36 will be constitutively and eliciting downstream GFP expression. Under low temperatures (below 36 degree), the pTlpa36 will be repressed, and drastically reducing GFP expression

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
    Illegal XhoI site found at 2035
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3303


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/control
Parameters
chassisE.coli (beta 10)