Part:BBa_K2255005

Domain 3 of p3 from M13 (Rfc25)

The molecular interactions that mediate the entry of filamentous phages into their hosts are mediated by the protein 3. This protein consists of three domains, separated by glycine-rich regions, that serve distinct roles in phage entry and release. The first two pIII domains, D1 and D2, are required for M13 adsorption and entry, while the third domain D3 is required for the assembly and release of M13 particles from host.

This part was designed with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which eases the assembly of multiple protein domains.

Usage and Biology

We used the protein D3 associated with multiple D1-D2 domains of p3 proteins coming from other filamentous phages to create phages that target various pathogenes. Thus, this biobrick was created to be associated with parts coming from our collection of attachment proteins. You can check out the first part of this collection: BBa_K2255008 and our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]