DNA

Part:BBa_K2429005

Designed by: Adil Yusuf, Nia Myrie   Group: iGEM17_MIT   (2017-10-08)
Revision as of 19:26, 8 October 2017 by Registry (Talk | contribs)

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pTRE 2-fluroescent protein (2-FP) reporter construct

This is a 3 exon, 2 intron report construct that allows use to test if we can splice together different protein isoforms conditionally. The output is the translation eYFP or eBFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal XbaI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal BamHI site found at 339
    Illegal BamHI site found at 1750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal XbaI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2620
    Illegal XbaI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None