Composite

Part:BBa_K2380025

Designed by: Thomas Georg Wagner   Group: iGEM17_TU_Darmstadt   (2017-10-08)
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Anderson Promoter BBa_J23100-NheI cleavage site-RBS B0034-BpiI recognition site

We took the well characterized parts BBa_J23100 (Anderson Promoter) and BBa_B0034 (RBS), and combined them to a new BioBrick. Thereby, downstream of the Anderson Promoter and upstream of the RBS sequence, we added an NheI cleavage site. In addition, a BpiI recognition site is located downstream of the RBS sequence. Our new part is an extension to our part BBa_K2380024 with which the RBS sequence of an existing composite part can be changed. BBa_K2380025 therefore provides a tool for changing the RBS sequence as well as the promoter enabling you to adjust both to your respective application. The NheI restriction site which which was inserted downstream of the Anderson Promoter generates stickyends that are complementary to the sticky ends XbaI and SpeI create. This enables the exchange of the promoter. In addition to the advantage of changing promoter and RBS of a consisting composite, you are able to add promoter and RBS in a single step.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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