Measurement

Part:BBa_K2406071

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-07)
Revision as of 14:46, 7 October 2017 by Registry (Talk | contribs)

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Rox-Term-Slox Measurement Construct

This part was designed to test if Rox and Slox Target sites were orthogonal. If either Dre or SCre recombinase could recognise both sites, it would catalyse recombination leading to expression of RFP. If the target sites were orthogonal, no RFP would be produced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 886
    Illegal AgeI site found at 998
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None