Measurement

Part:BBa_K2406051

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-09-29)
Revision as of 11:11, 7 October 2017 by JackSuitor (Talk | contribs)


Rox-Term-Rox Measurement construct.

Rox-Term-Rox construct inserted in BBa_J04450 part. If Dre recombinase is present it will cause recombination between the two rox sites, excising the terminator and allowing expression of the RFP protein. Therefore, this plasmid can be used as an assay for determining levels of Dre recombinase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 876
    Illegal AgeI site found at 988
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None