Regulatory
GST6 (shor

Part:BBa_K2404004

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)
Revision as of 11:54, 6 October 2017 by Gparry75 (Talk | contribs)


GST6 shortened - a salicylic acid-induced promoter

The GST6 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to Salicylic acid.

This promotor includes the sequence -407 > +90 in relation to the GST6 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the pSB1C3 plasmid using BsmBI.

The sequence includes an internal BsmBI site so was cloned into pSB1C3 by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794


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Parameters
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