Part:BBa_K2404001
A Thyroid Stimulating Hormone antagonist with His-Tags
This is a coding sequence of a gene that produces a protein that competitively inhibits thyroid stimulating hormone (TSH). This includes a 6xHis-tags to aid protein purification.
TSH was first generated and characterised by <a href="http://www.jbc.org/content/276/7/4543.long">Fares et al (2001) JBC 276, 4543-4558</a>. The protein includes two parts, the beta subunit from Thyrotropin (TSH) fused to a common alpha subunit shared by hormone glycoproteins. This is linked by a short CTD linker sequence. This TSH antagonist has been modified to remove glycosylation sites that exist on both subunits. As a result this antagonist binds to the TSH receptor but does not activate it.
TSH has been expressed in a murine expression system and shown to inhibit the auto-immune antibodies that cause in Graves' disease (<a href="http://www.pnas.org/content/113/5/1244.full">Saxena et al (2015). PNAS 113, 1244-129</a>).
We intend to use this TSH protein as a therapeutic treatment to treats hyperthyroidism in himans.
This part has been cloned ito pSB1C3 using BsmBI and conforms to the Phytobrick standard. It is flanked by BsaI sites together 5' aatg and 3' gctt cloning sequences for subsequent cloning into a level 1 plasmid.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 540
Illegal PstI site found at 582 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 540
Illegal PstI site found at 582 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 795
Illegal XhoI site found at 607 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 540
Illegal PstI site found at 582 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 540
Illegal PstI site found at 582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 827
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