Part:BBa_K2492001
CoCHR Channelrhodopsin
Channelrhodopsins are a subfamily of retinylidene proteins (rhodopsins) that function as light-gated ion channels[1]. CoCHR is a kind of microbial-type channelrhodopsin originated from Chloromonas oogama, which is an unique ion channel served as light switch.In our case, CoCHR is a cation-selective light sensor, which promptly generates a large permeability of divalent cations after absorption of photon from our excitation source. In order to investigate how olfactory messages are transmitted by the neutron systems and activate AWA in certain purpose without much delay, we express CoCHR in AWA through linkage to the cell-specific promoter odr-10. Thus when we shed the ~400nm excitation light to transgenic C.elegans which exist the cell-specific promoter for AWA, the activated AWA is able to cause obvious response in body twisting comparing to wild type C.elegans. Whether the certain neuron is activated is reported by another part called GEM-GECO, a calcium indicated for excitation visualisation. In short, we establish a light-sensed AWA controlling system, so as to using this part to manipulate the activation of AWA, establish the response to input stimulation. The merit of this part is sensitivity which is five-times more sensitive to blue light than formal ChR2. Thus the low intensity requirement avoids the cross-activation of Crimson, which is another transmitted channelrhodopsin in AWB. Moreover, in order to confirm the where the target neurons are and roughly estimate the expression level of CoCHR, we also construct a fashion reporter protein called mcherry.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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