Coding

Part:BBa_K2443043:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-04)
Revision as of 22:26, 4 October 2017 by Graeme.glaister (Talk | contribs) (→‎Source)


Asp RS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703


Design Notes

Codon optimized for use in Escherichia coli.



Source

Sequence obtained from: http://www.uniprot.org/uniprot/P21889 C-terminal His tag chosen because of the following publication: Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M., Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. ACS Synth. Biol. 2: 43-52

References