DNA

Part:BBa_K2308002

Designed by: Yunpeng Dai   Group: iGEM17_ECUST   (2017-10-04)
Revision as of 12:26, 4 October 2017 by Registry (Talk | contribs)

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sYFP2 knock In for fusion expression

This part is used to knock in the gene sYFP2 in Rhodobacter Sphaeroides, replacing the end coden of gene puhA, and sYFP2 will be expressed with H-subunit of light reaction center as fusion protein. The part is composed of 404bp of upstream DNA sequence(the end coden of puhA is not included) and 720bp coding sequence of sYFP2 (Part:BBa_K2308003) and 562bp of downstream DNA sequence. After homologous recombination, sYFP2 will be expressed with the H-subunit through fusion expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
    Illegal BamHI site found at 1217
    Illegal XhoI site found at 96
    Illegal XhoI site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 36
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 336
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None