Tag

Part:BBa_K2368024

Designed by: BIT-China 2017   Group: iGEM17_BIT-China   (2017-10-04)
Revision as of 06:06, 4 October 2017 by Hrlpl (Talk | contribs)


Introduction

His+T1R3 overlap

In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-6*His tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.

Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and His tag. And hope to show the location of T1R3 through immunofluorescence for detecting His tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 12
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 95
  • 1000
    COMPATIBLE WITH RFC[1000]


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