Composite

Part:BBa_K2206005:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)
Revision as of 22:58, 2 October 2017 by SLovat (Talk | contribs)


Toehold switch for hsa-miR-27b-3p with luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 936


Design Notes

No terminators are needed as the pSB1C3 plasmid contains terminators.


Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References