Device

Part:BBa_K2246000:Design

Designed by: JUAN CARLOS RUEDA SILVA   Group: iGEM17_TecCEM   (2017-10-02)
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BSLA: siRNA production and reporter cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 750
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The blue chromoprotein is used due to is visibility to the naked eye and its small size compared with other reporters. The promoter and terminator used work with T7 RNApol due to its high transcription rate. It has a BamHI and a HinCII cut sites around the region where the siRNA should be inserted, those enzymes cut sites were choosen, as they do not hybridize between each other, thus making the perfect form double stranded siRNA formation.


Source

Synthetic part.

References