Composite

Part:BBa_K2206005:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)
Revision as of 19:38, 2 October 2017 by Registry (Talk | contribs)

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Toehold switch for hsa-miR-27b-3p with luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 936


Design Notes

We ensured that there are no stop codons between the start codon and the protein coding sequence. This part contains an RBS


Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References