Composite

Part:BBa_K2282005

Designed by: Pauline Coudert   Group: iGEM17_IONIS-PARIS   (2017-10-02)
Revision as of 13:26, 2 October 2017 by Registry (Talk | contribs)

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BBa_K592009 (AmilCP) consitutive expression system.

AmilCP is expressed thanks to the Anderson constitutive promoter and translation is initiated at the ribosome binding site. AmilCP is a chromoprotein that has already been successfully used by several iGEM teams and leads to a strong color visible to the naked eye (The protein has an absorbance maximum at 588 nm) but also a temperature-dependent expression when used with a weak promoter. The double terminator enables robust termination of transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None