Part:BBa_K2368020

Introduction

BCP+T1R3 overlap

In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R3- amilCP (K592010). The part K592009 is blue chromoprotein and can exhibit strong blue color when expressed. Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and C-terminal of amilCP. And hope to show the location of T1R3 through fluorescence microscope for detecting amilCP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]