Introduction

500 bp of homologous arm＋His

Similarly, in order to knock out the gene called Sst2 which have the inhibition to the GpaI who play an important role in the signal transduction, The composite part is composed of the length of 500 bp of homologous arm and the marker of His, and it can replace the gene of Sst2 by homologous recombination, which can eliminate the influence of inhibition of sweetness signal transduction.