Part:BBa_K2368010

Introduction

myC+T1R3 overlap

In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-Myc tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.<p> <p>Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and Myc tag. And hope to show the location of T1R3 through immunofluorescence for detecting Myc tag.<p>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]