Composite

Part:BBa_K2423005

Designed by: Oscar Broström   Group: iGEM17_Uppsala   (2017-10-01)
Revision as of 18:44, 1 October 2017 by Registry (Talk | contribs)

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CaCCD2 with BBa_J04500

This BioBrick codes for the enzyme called CaCCD2 that is the first of three enzymes in the crocin pathway that starts from zeaxanthin. CaCCD2 catalyzes the reaction from zeaxanthin to crocetin dialdehyde. In nature the enzyme can be found in Crocus Sativus (the plant where saffron is harvested from).

This BioBrick contains a lac promoter which means that it has to be induced by lactose or some other closely related derivative, such as IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 837


[edit]
Categories
Parameters
None