RNA

Part:BBa_K2379002

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-09-30)
Revision as of 04:45, 1 October 2017 by Registry (Talk | contribs)

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RNA pHmeter with natural scar site

The RNA pHmeter is used for pH-sensitive translational regulation and initiates translation at pH 8.5. It is based on the RNA encoding gene, SraF from E.coli that is a part of the 5' UTR of the alx locus and brings about alx expression only under extreme alkaline conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 118


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Categories
Parameters
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