Generator

Part:BBa_K2239001:Design

Designed by: Ruohong Wang   Group: iGEM17_SDSZ-China   (2017-09-30)
Revision as of 16:25, 30 September 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ChBD-7alphaHSDH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1041
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 875


Design Notes

-


Source

The 7alpha-HSDH sequence is cloned from the genome of E.coli through PCR amplification, using the primers designed and synthesized based on its sequence. The restriction site BamH I is added to the upstream primer, and Hind III is added on the downstream primer. The CBD sequence is retrieved from the GeneBank, and is inserted into plasmid pUC57. The CBD gene is then cloned from the plasmid by PCR, with the restriction site Hind III added to the upstream primer, and Xhol I added to the downstream primer.

References