Tag

Part:BBa_K2255003:Design

Designed by: Camille Garcia   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 16:08, 29 September 2017 by Marlon (Talk | contribs)


Multi-Tag (Rfc 25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to create a multi-tag with a his-tag (attachment to Co or Ni column) and a streptavidine-tag (wich can bind biotin). Between those tag, we added a TEV cutting site to give the possibility to decrease the length of this multi-tag.

Our tag is composed of StrepTag-TEV-6His, which can be constructed as GSG-WSHPQPEL-GSG-ASQFYLNE-GSG-HHHHHH

Glycine and serine residues are added to the sequence in order to give flexibility and space between the tags and the cutting site.

We retro-translated this sequence and optimized it for E. coli.

Source

This part is made with the consensus sequence of the streptavidine tag, the histidine tag and the TEV protease cutting site.

References