Measurement

Part:BBa_K2406053

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-09-29)
Revision as of 11:07, 29 September 2017 by Registry (Talk | contribs)

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Vox-Term-Vox Measurement Construct

Vox-Term-Vox construct inserted in BBa_J04450 part. If Vika recombinase is present it will cause recombination between the two Vox sites, excising the terminator and allowing expression of the RFP protein. Therefore, this plasmid can be used as an assay for determining levels of Vika recombinase activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 880
    Illegal AgeI site found at 992
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None