Composite

Part:BBa_K2332003

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-09-29)
Revision as of 10:28, 29 September 2017 by Registry (Talk | contribs)

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tRNA-Pyl (pylT)

This composite part encodes tRNA-Pyl (pyIT), a tRNA with the anticodon CUA able to incorporate the photocaged amino acid pyrrolysine, into proteins in E. coli.

Pyrolysyl-tRNA synthetase first loads the tRNA with pyrrolysine present in the media. The charged pyIT can then incorporate pyrrolysine into proteins where the corresponding sense codon TAG, is present in the mRNA during translation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None