Coding

Part:BBa_K2450101:Design

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-09-28)
Revision as of 13:27, 28 September 2017 by Registry (Talk | contribs)

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TEV protease tagged with mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1444
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to be able to purify TEV with and without the mCherry, so we had the His tag on the other terminus of the protein.


Source

mCherry from AddGene. TEV protease from xxx

References