Primer

Part:BBa_K2309032

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-27)
Revision as of 03:26, 28 September 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Standard reverse primer for Gibson Assembly to pNZ8148

This primer contain 17 bp pf BioBrick suffix and the rest for Gibson Assembly. PstI is used to linearize pNZ8148.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None