Coding

Part:BBa_K2450201

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-09-27)
Revision as of 13:48, 27 September 2017 by Registry (Talk | contribs)

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TetR with TEV cleavage site and CFP tag

This part is a modified TetR repressor protein containing a cleavage site for TEV protease. It offers a new way of relieving repression of a DNA system. The cleavage site is located in the linker between the DNA binding domain and dimerisation domain of TetR. It also has a His-tag for easy purification in a nickel column. It is an improvement of a previous iGEM part.

The TetR repressor is only functional as a dimer. Each monomer has a DNA-binding domain and a dimerisation domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None