Coding
Part:BBa_K2450201
Designed by: Zoe Ford Group: iGEM17_Oxford (2017-09-27)
TetR with TEV cleavage site and CFP tag
This part is a modified TetR repressor protein containing a cleavage site for TEV protease. It offers a new way of relieving repression of a DNA system. The cleavage site is located in the linker between the DNA binding domain and dimerisation domain of TetR. It also has a His-tag for easy purification in a nickel column. It is an improvement of a previous iGEM part.
The TetR repressor is only functional as a dimer. Each monomer has a DNA-binding domain and a dimerisation domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |