Coding

Part:BBa_K2201200:Design

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-09-23)
Revision as of 20:33, 23 September 2017 by Registry (Talk | contribs)

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Tyrosyl tRNA/aminoacyl-synthetase for the incorporation of 2-nitrophenylalanine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1153
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 404
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Remove the TyrRS and replace with the 2-NPA-RS.


Source

Standard M.jannaschii TyrRS plasmid.

References