Coding

Part:BBa_K2201200

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-09-23)
Revision as of 20:33, 23 September 2017 by Registry (Talk | contribs)

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Tyrosyl tRNA/aminoacyl-synthetase for the incorporation of 2-nitrophenylalanine

To get the noncanonical amino acid 2-nitrophenylalanine (2-NPA) incorporated into a protein auch as the fusion protein of K2201321, a sequence with a TAG is needed along with an additional tRNA synthetase (RS) that is able to load the ncAA to the tRNA. The ribosome is then able to incorporate this amino acid at an amber codon if the target protein with the amber codon and a plasmid with the 2NPA-RS and tRNA are co-transformed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1153
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 404
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1645
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None