Regulatory
WRKY30

Part:BBa_K2404005

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)
Revision as of 17:37, 22 September 2017 by Ryancoates (Talk | contribs)


WRKY30 - a promoter induced by the presence of cellulose-derived oligomers

This is an inducible promoter that has been isolated from Arabidopsis thaliana . It responds to the presence of cellulose-derived oligomers in the cell, as it is a ‘Damage Associated Molecular Patterns’ (DAMP) responsive promoter. This part is suitable for Golden Gate cloning using type IIS restriction enzymes. This part is contained in the pSB1C3 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794


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Categories
Parameters
None