Composite
Kise Ryota
Part:BBa_K2200006
Designed by: Meredith Eating Xiang Group: iGEM17_Shenzhen_SFLS (2017-07-22)
Revision as of 04:42, 22 September 2017 by EatingXiang625 (Talk | contribs)
pU6+sgRNA+pHEf1A+hCas9
This part is consisted of Human U6 promoter, sgRNA that targets a specific BRAF sequence. With its high flexibility of being devised to target different tumor gene sequences, it has a great market upon innovating cancer treatments since it will revolutionize this market by personalizing the remedy.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5117
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 863
Illegal XhoI site found at 1262 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 997
Illegal NgoMIV site found at 4214
Illegal NgoMIV site found at 5123
Illegal AgeI site found at 375 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 5242
Illegal SapI.rc site found at 2633
Illegal SapI.rc site found at 2875
[edit]
Categories
Parameters
None |