Composite
Kise Ryota

Part:BBa_K2200006

Designed by: Meredith Eating Xiang   Group: iGEM17_Shenzhen_SFLS   (2017-07-22)
Revision as of 04:42, 22 September 2017 by EatingXiang625 (Talk | contribs)


pU6+sgRNA+pHEf1A+hCas9

This part is consisted of Human U6 promoter, sgRNA that targets a specific BRAF sequence. With its high flexibility of being devised to target different tumor gene sequences, it has a great market upon innovating cancer treatments since it will revolutionize this market by personalizing the remedy.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
    Illegal XhoI site found at 1262
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
    Illegal NgoMIV site found at 4214
    Illegal NgoMIV site found at 5123
    Illegal AgeI site found at 375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5242
    Illegal SapI.rc site found at 2633
    Illegal SapI.rc site found at 2875


[edit]
Categories
Parameters
None