Reporter

Part:BBa_K2492000

Designed by: ZHOU Xinyu   Group: iGEM17_SUSTech_Shenzhen   (2017-09-21)
Revision as of 12:02, 21 September 2017 by Registry (Talk | contribs)

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GFP (optimised, for use in the C. elegans chassis)

Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group we use it to locate the neuron AWB of C.elegans. If we get successfully microinjection C.elegans, GFP will specifically high expressed in AWB with the help of special promoter str1(no biobrick part).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797


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Categories
Parameters
None