Reporter

Part:BBa_K2205002:Design

Designed by: Bradley Brown   Group: iGEM17_Newcastle   (2017-09-21)
Revision as of 10:17, 21 September 2017 by Registry (Talk | contribs)

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J23100-deGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed as a gBlock and Gibson assembled into pSB1C3 using HiFi assembly. Overhangs were designed to esnure that the biobrick prefix and suffix were re-generated.


Source

Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. Shin J, Noireaux V. J Biol Eng. 2010 Jun 24;4:8. 10.1186/1754-1611-4-8 PubMed 20576148

References