Regulatory
Switch

Part:BBa_K2329000

Designed by: Karl Alex Hedin   Group: iGEM17_Chalmers-Gothenburg   (2017-09-19)
Revision as of 14:44, 19 September 2017 by AlexHedin (Talk | contribs)


A non-reversible switch

The promotor TEF1 within two mutated LoxP-sites. The mutation in the LoxP-sites introduce a non-reversible switch. This switch-system make it possible to switch the direction of the promotor, and so on encoding different genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 275


[edit]
Categories
Parameters
None