Reporter

Part:BBa_K2269005

Designed by: José Álvaro Ballesteros González   Group: iGEM17_Valencia_UPV   (2017-09-10)
Revision as of 07:44, 11 September 2017 by Albagoutiel (Talk | contribs)


Register Assembly 2/3 (Pr-1a)

Part of a red light-controlled synthetic genetic switch. Designed module for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence (Müller et al., 2014).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 54
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 54
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None