Regulatory

Part:BBa_K2215004

Designed by: Megan Sare   Group: iGEM17_ULaVerne_Collab   (2017-09-11)
Revision as of 06:58, 11 September 2017 by Registry (Talk | contribs)

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Putative Symbiodinium Promoter

This is putative Symbiodinium promoter that is believed to work best in Symbiodinium due to its unique features, such as a "TTTT-box rather than a TATA box, a unique transcription start site branch point, and acceptor for the dinoflagellate spliced leader". With this promoter, it should allow for more expression and stability of transgenes introduced in Symbiodinium.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 971
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal SpeI site found at 972
    Illegal PstI site found at 986
    Illegal NotI site found at 12
    Illegal NotI site found at 979
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal BglII site found at 360
    Illegal BglII site found at 438
    Illegal BglII site found at 790
    Illegal XhoI site found at 442
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6
    Illegal suffix found in sequence at 972
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6
    Illegal XbaI site found at 21
    Illegal SpeI site found at 972
    Illegal PstI site found at 986
    Illegal AgeI site found at 308
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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