Device

Part:BBa_K2483002

Designed by: Bryan Nowack   Group: iGEM17_Potsdam   (2017-09-07)
Revision as of 12:58, 7 September 2017 by Registry (Talk | contribs)

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Lac regulated dCas9 with LacI constitutively expressed

This part is designed to produce dCas9 when Lac or IPTG is present. LacI-LVA (BBa_C0012) is constitutively expressed under the pRep4 promoter and dCas9 has the Lac promoter (BBa_R0010) at the 5'-end.

This part was used in our metabolic channelling project and is also part of BBa_K2483004, our final construct for our experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1258
    Illegal BamHI site found at 4945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr/cas9
Parameters
chassisE.coli JM109
proteinsLacI and dCas9