DNA
TaC1-6bp

Part:BBa_K2483005:Design

Designed by: Sophia Borowski   Group: iGEM17_Potsdam   (2017-08-30)
Revision as of 08:28, 6 September 2017 by BryanN (Talk | contribs)

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sgRNA target site couples facing each other with 6 bp spacer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 562
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To guarantee that both sgRNAs face each other, we had to make one sgRNA bind to the opposite strand. We also have big spacer of 118 bp inbetween the target ste couples to reduce homologous recombination. The recoginition sequences were analyzed with an online tool for efficacy.


Source

The sequence is purely synthetic.

References