Coding

Part:BBa_K2255007

Designed by: Camille Garcia   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 13:14, 30 August 2017 by Kamy (Talk | contribs)


Signal sequence of p3 from M13 (Rfc25)

The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use E. coli to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.

This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None