Coding

Part:BBa_K2255010

Designed by: Camille Garcia   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 12:59, 30 August 2017 by Kamy (Talk | contribs)


p3_P.aeruginosa (Rfc25)

This part is sequence coding for the D1 and D2 of the protein 3 of Pf3, a filamentous phage that target P.aeruginosa. Those domains are required for phage adsorption and entry.

This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 814


[edit]
Categories
Parameters
None