Coding

Part:BBa_K2255006:Design

Designed by: Hussein Daher   Group: iGEM17_Aix-Marseille   (2017-08-28)
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EPS-Depolymerase (Rfc25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein is 852 amino acid with is translated to 2556 nucleobases is quite big making it difficult to work with so a process of trimming was donne. Seeing as the domain we are interested in pectin lyase start from the 139th to 426th amino acid it is possible to remove the excess amino acids without harming the functionality and specificity of the enzyme.


Source

Genomic sequence from the phage vB_XveM_DIBBI

References