Coding

Part:BBa_K2255005

Designed by: Camille Garcia   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 13:27, 28 August 2017 by Kamy (Talk | contribs)


Domain 3 of p3 from M13 (Rfc25)

The molecular interactions that mediate the entry of filamentous phages into their hosts are mediated by protein 3. This protein consist of three domains, separated by glycine-rich regions, that serve distinct roles in phage entry and release. The first two pIII domains, D1 and D2, are required for M13 adsorption and entry, while the third domain D3 is required for the assembly and release of M13 particles from host.

We used the protein D3 to associated it with multiple D1-D2 domains of p3 protein coming from other filamentous phages in order to create phage that target various pathogenes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None