Composite

Part:BBa_K2423000:Design

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)
Revision as of 07:26, 18 August 2017 by Registry (Talk | contribs)

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CaCCD2 under the control of a constitutive promoter (BBa_J23106)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 672


Design Notes

We made a complete device including promoter and RBS. We also included a 6x his tag for easy purification of the expressed protein.


Source

Crocus ancyrensis

References