Composite

Part:BBa_K2197300

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-08-11)
Revision as of 07:43, 11 August 2017 by Registry (Talk | contribs)

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Expression of GFP under the control of a uric acid concentration-sensitive promoter device

Engineered e.coli encodes part BBa_K2197300. By adding blood samples to culture medium which engineered e.coli is cultured, e.coli expresses different level of GFP. By analysing the GFP level with HPLC, the uric acid concentration of the sample can be estimated. This part ensures a rapid detection of uric acid concentration thus gout.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1098
    Illegal NheI site found at 1121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 291
    Illegal BsaI.rc site found at 1814
    Illegal SapI.rc site found at 255


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Parameters
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