Reporter

Part:BBa_J100324

Designed by: Deborah Thurtle-Schmidt   Group: Campbell M Lab   (2017-08-02)
Revision as of 20:20, 2 August 2017 by Macampbell (Talk | contribs)

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repClone Red minus TT

This is the same part as J100205 except the TT has been removed. We found J100205 to be unstable and J100324 part is more stable, it grows better, and yields more plasmid than J100205.

We designed repClone Red (J100205) to test the function of repressors. Internal BsaI sites allow the insertion of the Ptet promoter and removal of the backwards P2 promoter pointed towards GFP. Successful cloning of the Ptet promoter will change colonies from green to not green. Variations on the Ptet promoter, or introduction of anhydrotetrocycline (aTc), will affect the activity of the repressor, as indicated by the fluorescence of colonies containing the plasmid. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.

J100324.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2219
    Illegal AgeI site found at 2331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1569
    Illegal BsaI.rc site found at 1517


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